Propionic Rats and Autism

Protective and therapeutic potency of N-acetyl-cysteine on propionic acid-induced biochemical autistic features in rats

http://www.jneuroinflammation.com/content/pdf/1742-2094-10-42.pdf

Background

The investigation of the environmental contribution for developmental neurotoxicity is very critical.

Many environmental chemical exposures are now thought to contribute to the development of neurological disorders, especially in children. Results from animal studies may guide investigations of human populations towards identifying either environmental toxicants that cause or drugs that protect from neurotoxicity and may help in treatment of neurodevelopmental disorders.

Objective

To study both the protective and therapeutic effects of N-acetyl cysteine on brain intoxication induced by propionic acid (PPA) in rats.

Methods

Twenty-eight young male Western Albino rats were enrolled in the present study. They were grouped into four equal groups, each of 7 animals. Group 1: control group, orally received only phosphate buffered saline; Group 2: PPA-treated group, received a neurotoxic dose of of PPA of 250 mg/kg body weight/day for 3 days; Group 3: protective group, received a dose of 50 mg/kg body weight/day N-acetyl-cysteine for one week followed by a similar dose of PPA for 3 days; and Group 4: therapeutic group, treated with the same dose of N-acetyl cysteine after being treated with the toxic dose of PPA. Serotonin, interferon gamma (IFN-gamma), and glutathione-s-transferase activity, together with Comet DNA were assayed in the brain tissue of rats in all different groups.

Results

The obtained data showed that PPA caused multiple signs of brain toxicity as measured by depletion of serotonin (5HT), increase in IFN-gamma and inhibition of glutathione-s-transferase activity as three biomarkers of brain dysfunction. Additionally Comet DNA assay showed remarkably higher tail length, tail DNA % damage and tail moment. N-acetyl-cysteine was effective in counteracting the neurotoxic effects of PPA.

Conclusions

The low dose and the short duration of N-acetyl-cysteine treatment tested in the present study showed much more protective rather than therapeutic effects on PPA-induced neurotoxicity in rats, as there was a remarkable amelioration in the impaired biochemical parameters representing neurochemical, inflammatory, detoxification and DNA damage processes.

The complete article is available as a provisional PDF. The fully formatted PDF and HTML versions are in production.

This entry was posted in Autism, Environment, Immune System, Inflammation, Mice, Neurology, Physiology, Treatment. Bookmark the permalink.

1 Response to Propionic Rats and Autism

  1. Roger Kulp says:

    I know organic acidemias are a common feature of mitochondrial disease,and propionic academia is probably the most common one of these.

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